Individuals wanting to attend a tutorial must be registered with the CPI 2008 conference. Please complete your tutorial registration form and submit it once you have completed your conference registration.

Tutorial registration is now closed.

For questions contact info@marketwhys.ca

As part of its educational mandate, CPI offers two post/pre-conference workshops focusing on themes of interest to trainees and other researchers. These workshops provide an interactive environment for people working in proteomics areas to enhance their knowledge through in-depth, focused tutorials taught by experts in the field. To facilitate an optimal learning environment, registration is limited to 10 students per workshop.

The tutorials will be given on Thursday, May 1, and Friday, May 2, 2008, before the CPI conference. The tutorial registration is open. You can choose different sessions (up to two, either day 1 or day 2) from different tutorials (maximum two). Space is limited to 10 students per workshop, so register early. The fees are $157.50 ($150.00+$7.50 GST) for one day and $315.00 for two days($300.00+$15.00 GST).

The tutorials include:

Tutorial 1

Sample Preparation, Enrichment and Separation for Protein Analysis (Registration Closed);
(Foster, UBC)

Date: Thursday, May 1 and Friday May 2
Hours: 9:00 a.m. - 3:30 p.m.
Location: 425-2125 East Mall, University of British Columbia, Vancouver, BC
*Cost: $150.00 + $7.50 GST = $157.50 (for one day)
          $300.00 + $15.00 GST = $315.00 (for two days)
Maximum: 10 People

This hands-on workshop will focus on methods for sample preparation, sample enrichment, sample cleanup and sample separations up front of LC-MS/MS analyses. We will cover protein precipitation, in solution and in-gel digestion of proteins, TiO2 enrichment of phosphopeptides, strong cation exchange (SCX) and in solution isoelectric focusing of proteins and peptides and, finally, peptide desalting/cleanup with STop And Go Extraction (STAGE) tips.

Day 1 - Tutorial Content Overview

Sample Preparation, Enrichment and Separation for Protein Analysis:
- Protein Precipitation
- In Solution Digestion
- In Gel Digestion
- In Solution Isoelectric Focusing

Day 2 - Tutorial Content Overview

Sample Preparation, Enrichment and Separation for Protein Analysis:
- In Gel Digestion
- SCX Fractionation
- Sample Cleanup

Tutorial 2

Protein Characterization and Identification Workshop (Registration Closed);
(Kast, UBC)

Date: Thursday, May 1 and Friday May 2
Hours: 9:00 a.m. - 3:30 p.m.
Location: The Biomedical Research Centre, 2222 Health Sciences Mall, University of British Columbia, Vancouver, BC
*Cost: $150.00 + $7.50 GST = $157.50 (for one day)
          $300.00 + $15.00 GST = $315.00 (for two days)
Maximum: 10 People

This hands-on workshop will address various workflows for protein identification and characterization. Topics will include accurate molecular weight determination of proteins, in-gel and in-solution digestion, peptide mass fingerprinting and LC-MS/MS-based peptide sequencing using on-line and off-line strategies and different instrument geometries, post-translational modification mapping, data extraction and interpretation, and in-silico analysis using different sequence databases and data repositories.

Day 1 - Tutorial Content Overview

Morning:
- Introduction
- Intact protein analysis (MALDI and ESI)

Afternoon:
- In-gel and in-solution digestion
- Peptide mass fingerprinting

Day 2 - Tutorial Content Overview

Morning:
- LC-MS/MS and peptide sequencing (on-line for ESI and off-line for MALDI)
- Data extraction

Afternoon:
- Data interpretation
- In-silico analysis (sequence databases, data repositories)
- Post-translational modification mapping

Tutorial 3

High-field FTICR-MS for Life Sciences (Registration Closed);
(Borchers, UVic - GBC Proteomics Centre)

Date: Thursday, May 1 and Friday May 2
Hours: 9:00 a.m. - 3:30 p.m.
Location: UVic - GBC Proteomics Centre, #3101-4464 Markham Street, Vancouver Island Technology Park, Victoria, BC
*Cost: $150.00 + $7.50 GST = $157.50 (for one day)
          $300.00 + $15.00 GST = $315.00 (for two days)
Maximum: 10 People

This tutorial focuses on FTICR-MS based methods and approaches for top-down and bottom-up proteomics, analysis of non-covalent protein complexes and metabolomics. We will provide hands-on training in different FTICR-MS techniques including LC-MS/MS for metabolite and peptide analysis. Numerous dissociation and combination techniques such as CID, ECD, and SORI will be described for detailed analysis of intact proteins and other bio-molecules. Novel protein separation approaches like FFE and sample preparation methods for metabolomics and native protein complexes will also be addressed.

Day 1 - Tutorial Content Overview

High-field FTICR-MS for life Sciences (Borchers, UVic-GBC Proteomics Centre)
- Top-down Proteomics
- Analysis of non-covalent complexes
- Metabolomics via direct infusion

Day 2 - Tutorial Content Overview

High-field FTICR-MS for life Sciences (Borchers, UVic-GBC Proteomics Centre)
- LC-MS/MS for Proteomics and Metabolomics

Tutorial 4

Quantitative Proteomics (Registration Closed);
(Borchers, UVic - GBC Proteomics Centre)

Date: Thursday, May 1 and Friday May 2
Hours: 9:00 a.m. - 3:30 p.m.
Location: UVic - GBC Proteomics Centre, #3101-4464 Markham Street, Vancouver Island Technology Park, Victoria, BC
*Cost: $150.00 + $7.50 GST = $157.50 (for one day)
          $300.00 + $15.00 GST = $315.00 (for two days)
Maximum: 10 People

An intensive hands-on training tutorial on MS-based approaches for unbiased quantitative protein analysis in biological samples including iTRAQ labeling followed by 2D peptide separation and direct techniques such as ESI and MALDI-MS/MS (LC-MALDI). Targeted quantitation will be demonstrated with the MRM technology using both ESI- and MALDI-QTrap instruments. This tutorial also covers incorporation of isotopically-labeled peptides as internal standards for absolute quantitation. Furthermore, the course will provide an overview of combined affinity enrichment methods and MS, such as SISCAPA and iMALDI, for quantitation of low abundant proteins.

Day 1 - Tutorial Content Overview

Quantitative Proteomics (Borchers, UVic-GBC Proteomics Centre):
- Lecture about MRM, iTRAQ, ICPL and label free quantitation
- Sample preparation for quantitative proteome analysis
- MRM analysis for absolute quantitation

Day 2 - Tutorial Content Overview

Quantitative Proteomics (Borchers, UVic-GBC Proteomics Centre):
- iTRAQ analysis by LC-MS/MS using ESI and MALDI

*Cost for participation includes tutorial notes and lunch. Travel and accommodation to the tutorial venue is the responsibility of the participant. Please note these sessions are held in conjunction with the CPI 2008 Conference. Participants must also be registered for the conference in order to attend the optional pre-conference tutorials.

If you have questions regarding the tutorials, please contact Steven Leard.